Part:BBa_K112624:Design

{Pbad}.{rbs_pelB>}.{} in BBb

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1317
  Illegal NgoMIV site found at 1540
  Illegal NgoMIV site found at 1987
  Illegal AgeI site found at 1023
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1005
  

Design Notes

N/A

Source

This part was made using AKL41, which consisted of {Pbad}.{rbs_pelB>}.{<phoA!}.{<b1006>}. The stop codon of the phoA region and the <b1006> terminator were removed.

PCR with ca998 Forward oligo, and mea37 reverse oligo for {<phoA>}.